Which Of The Following Should Be Chosen For Best Yield If One Were To Produce A Recombinant Protein In Large Amounts

Which of the following should be chosen for best yield if one were to produce a recombinant protein … continuous culture system (d)…

Answers:

(c)
• After having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein one has to consider producing it on a large scale. Small volume cultures cannot yield appreciable quantities of products.
• To produce in large quantities the development of bioreactors, where large volume (100-1000 litres) of culture (continuous) can be processed, was required.